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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in several tumors, which leads to chromosome instability, and thus may lead to tumorigenesis. This protein was discovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial purpose in endorsing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two supplemental splice variants ended up described but thorough nucleotide sequence details isn't nonetheless offered.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guidebook outlines even more achievable optimization of cellular and immunoassay parameters to make sure the very best outcomes are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the best possible benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope over the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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